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各位大侠:最近做原核表达,但转化涂板后总是不张菌,不知是怎么回事,我都做了很多遍了。我的片段大小是1.9kb,载体为PET32A,我用的BamHI和SaLI两种酶的,然后将酶切后的目的片段(6ul),酶切后的载体(2ul),T4连接酶1ul,buffer 1ul连接于16度过夜连接后,转到BL21菌中的,可是为什么总是张不出菌呢?我很着急,,请哪位 ...
题目:求助文献4篇篇号:1杂志全名及年卷期: J Mol Neurosci. 2007 Sep;33(1):25-31文题:The fatty acid transport protein (FATP) family: very long chain acyl-CoA synthetases or solute carriers?作者:Jia Z, Pei Z, Maiguel D, Toomer CJ, Watkins PA.PMID: 17901542篇号:2杂志全名及年卷期: ...
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